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KMID : 0545119990090010106
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 1 p.106 ~ p.112
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Newly Synthesized Phosphodiesterase 4(PDE4)Inhibitor, DWP205505,Inhibits TNF-α Secretion and mRNA Expression
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Lee, Suk Kyeong
Lee, Sun A/Byun, Hye Sin/Cho, Mi La/Kim, Wan Uk/Park, Sung Hwan/Cho, Chul Soo
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Abstract
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Intestinal bacteria comprise one-third of the contents of the large intestine in humans. Their interactions with the gastrointestinal immune system induce characteristic immunological responses which stimulate or suppress the host´s defense system. RAW 264.7 murine cell line was used as a macrophage model to assess the effects of the exposure to the isolated human intestinal bacteria. Bacteroides, Bifidobacterium, Eubacteruim, Sireprococcus and F. colt, on NO (nitric oxide). H,O. (hydrogen peroxide), and cytokines Ⅱ. (interleukin)-6 and TNF (tumor necrosis factor)-α production. RAW 264.7 cells were cultured in the presence of heat-killed bacteria for 24 h at concentrations of 0∼50 ㎍/㎖. Our results showed that Bueteroides and E. coli stimulated Ⅱ∼6. TNF-α, NO, and HO production at high levels even at IⅠ㎍/㎖, whereas Bifidobacterium, Eubacterium, and Streptococcus showed a low level of stimulation at Ⅰ ㎍/㎖ and a gradual increase as the cell concentration increased up to 50 ㎍/㎖. This result suggests that gram-negative Bacteroides and E. coli are better able to stimulate macrophage than gram-positive bifidobacterium Streptococcus, and Eubacterium. The in vitro approaches employed here should be useful in further characterization of the effects of intestinal bacteria on gastrorintestinal and systemic immunity.
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